Clontech Laboratories, a subsidiary of Takara Bio, has launched an RNA sequencing kit for samples that are compromised or do not have polyadenylated mRNA.
The Smarter universal low input RNA kit for sequencing combines the company's patented switching mechanism for ds cDNA synthesis with random priming, enabling transcriptome sequencing (RNA-Seq) from degraded total RNA samples.
Sequenceable RNA samples can be acquired via common sample preparation techniques such as formaldehyde fixed paraffin embedded tissue (FFPE) and laser capture microscopy (LCM).
In addition to reducing handling of the RNA sample, the SMART protocol minimises the risk of sample loss and preserves the original message.
Clontech Laboratories general manager Carol Lou said the company has already pushed the limits of RNA-Seq down to the single cell level with its Smarter ultra low RNA kit for illumina sequencing.
"Today, we are expanding the applications of Smart technology to include cDNA synthesis from samples that contain degraded RNA (eg FFPE or LCM samples) or non-polyadenylated RNA. We expect the Smarter universal low input RNA kit for sequencing to be a welcome addition to RNA-Seq research," Lou said.
The company is also planning to further expand the utility of the Smarter kits for NGS by combining them with other key technologies, including multiplex library preparation.