10X Genomics. has filed a patent for methods and compositions involving catalytic de-crosslinking of biological samples for in situ analysis. The claim outlines a method for sample analysis using a specific catalyst formula. GlobalData’s report on 10X Genomics gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on 10X Genomics, ChIP-seq sequencing was a key innovation area identified from patents. 10X Genomics's grant share as of January 2024 was 23%. Grant share is based on the ratio of number of grants to total number of patents.

In situ analysis method using catalytic de-crosslinking

Source: United States Patent and Trademark Office (USPTO). Credit: 10X Genomics Inc

A recently filed patent (Publication Number: US20240035071A1) discloses a method for sample analysis involving a biological sample immobilized on a substrate. The method includes contacting the biological sample with a catalyst that catalyzes de-crosslinking of molecular crosslinks in the sample, with the catalyst being a specific compound as defined by formula (I). The molecular crosslinks are products of one or more crosslinking agents, such as aldehydes, including formaldehyde. The catalyst may consist of various compounds selected from a specific group. Additionally, the method involves using a solution or suspension containing the catalyst and a buffer, which may include sodium dodecyl sulfate (SDS), urea, and/or a proteinase.

Furthermore, the method includes the use of a labelling agent with a binding moiety, such as an antibody, or a detectable label. The process also involves a reporter oligonucleotide with a barcode sequence. The biological sample is analyzed for protein analytes, which can be intracellular, membrane-bound, or extracellular proteins. The optical signal generated during the analysis is detected in situ, with imaging techniques like fluorescent microscopy being utilized. Additionally, the biological sample is probed to identify the protein analyte associated with the labelling agent, either directly or indirectly through intermediate probes with barcode sequences. The method offers a comprehensive approach to sample analysis, particularly in identifying and studying various protein analytes within biological samples.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies