Bio-Techne has filed a patent for a method of removing RNA from a sample using sodium metasilicate. The method is useful for preparing samples for DNA in situ hybridization assays. GlobalData’s report on Bio-Techne gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Bio-Techne, Peptide pharmacophores was a key innovation area identified from patents. Bio-Techne's grant share as of September 2023 was 40%. Grant share is based on the ratio of number of grants to total number of patents.
Method for removing rna from a sample
A recently filed patent (Publication Number: US20230313171A1) describes a method for removing RNA from a sample using sodium metasilicate. The method is particularly applicable to biological samples, including cultured cells, tissue specimens (such as formalin-fixed paraffin-embedded tissue), blood samples, and cytological samples.
The method involves contacting the sample with an aqueous solution of sodium metasilicate, with a concentration ranging from about 50 mM to about 200 mM, and a pH of about 12 to about 14. The sample can be heated to a temperature of about 35°C to about 45°C, for a duration of about 30 minutes to about 60 minutes. After the contacting step, the sample can be washed and a target DNA in the sample can be detected, for example, using DNA in situ hybridization.
Importantly, the method does not significantly alter the morphology of the sample, and it can reduce RNA levels by at least 90%. The RNA removed from the sample primarily comprises mRNA.
The patent also describes a composition comprising sodium metasilicate and a sample containing cells, as well as a kit comprising sodium metasilicate and one or more probes or reagents for detecting a target DNA in a sample. The kit may also include instructions for carrying out a DNA in situ hybridization assay.
In summary, the patent presents a method, composition, and kit for effectively removing RNA from various types of biological samples using sodium metasilicate. The method allows for the detection of target DNA in the sample while preserving the sample morphology. The kit provides the necessary components and instructions for carrying out the RNA removal and DNA detection process.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
