10X Genomics has developed a method for generating nucleic acid barcode molecules by coupling them to supports. The process involves assembling barcode molecules using ligation reactions, useful for analyzing nucleic acids, proteins, and perturbation agents. The patented method ensures efficient digestion of off-target products while preserving target products. GlobalData’s report on 10X Genomics gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on 10X Genomics, ChIP-seq sequencing was a key innovation area identified from patents. 10X Genomics's grant share as of January 2024 was 23%. Grant share is based on the ratio of number of grants to total number of patents.

Method for generating nucleic acid barcode molecules

Source: United States Patent and Trademark Office (USPTO). Credit: 10X Genomics Inc

A recently granted patent (Publication Number: US11884962B2) discloses a method for generating nucleic acid barcode molecules. The method involves providing a plurality of nucleic acid molecules coupled to a support, coupling additional nucleic acid molecules to assemble a variety of nucleic acid barcode molecules, and digesting off-target products with an exonuclease. The target products contain an exonuclease-resistant bond, with less than 50% of these bonds being digested by the exonuclease.

The patent claims also detail the use of specific exonucleases such as Exo1 exonuclease, Exo111 nuclease, or heat-labile DNAse in the method. Additionally, the method specifies the absence of reducing agents like dithiothreitol during the digestion process. The temperature range for the digestion step is set between 37 to 65 degrees Celsius for a duration of 15 minutes to 1 hour. Furthermore, the support for the nucleic acid molecules can be a bead, specifically a gel bead, with the digestion process not affecting the integrity of the bead. The method also involves coupling capture sequences to the nucleic acid molecules during the assembly of the barcode molecules.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.