Abcam has filed a patent for a method of detecting target nucleic acid. The method involves using hydrogel particles with capturing probes to capture the target nucleic acids in a sample. The captured nucleic acids are then separated from the particles and amplified with a detectable entity. The amplified nucleic acids are then recaptured by a second set of hydrogel particles with re-capturing probes. The presence and/or abundance of a detectable signal indicates the presence and/or abundance of the target nucleic acids in the sample. GlobalData’s report on Abcam gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Abcam, CRISPR nucleases was a key innovation area identified from patents. Abcam's grant share as of June 2023 was 1%. Grant share is based on the ratio of number of grants to total number of patents.
Method of detecting target nucleic acid using hydrogel particles
A recently filed patent (Publication Number: US20230193352A1) describes a method for detecting target nucleic acids using hydrogel particles. The method involves several steps to capture, amplify, and detect the target nucleic acids in a sample.
In the first step, a sample is contacted with a set of hydrogel particles that have capturing probes attached to them. These capturing probes have target capturing sequences that allow them to capture the target nucleic acids present in the sample. The captured target nucleic acids are then separated from the hydrogel particles.
Next, the captured target nucleic acids are amplified in a reaction mixture that contains a detectable entity. This amplification process labels the target nucleic acids with the detectable entity. The amplification product is then incubated with a second set of hydrogel particles that have re-capturing probes embedded throughout their matrix. These re-capturing probes recapture the amplified target nucleic acids.
The presence and/or abundance of the detectable signal generated by the detectable entity associated with the re-captured amplified target nucleic acids indicates the presence and/or abundance of the target nucleic acids in the sample. The method can be used to detect various types of target nucleic acids, such as microRNAs, mRNAs, non-coding transcripts, genomic DNA, cDNAs, siRNAs, DNA/RNA chimera, or a combination thereof.
The patent also describes the use of adapters to couple to the captured target nucleic acids and primers attached to a substrate for performing a polymerase chain reaction (PCR) to amplify the captured target nucleic acids. The signal generated by the detectable entity can be detected using a flow cytometer or an array scanner.
The patent further includes a method for detecting target nucleic acids using hydrogel particles, where the particles have encoding regions that bear detectable moieties to identify the capturing or re-capturing probes. The presence and/or abundance of the detectable signal generated by the detectable entity associated with the re-captured amplified target nucleic acids on the second set of particles indicates the presence and/or abundance of the target nucleic acids in the sample.
Additionally, the patent describes a kit for detecting target nucleic acids, which includes particles with probe regions bearing probes and encoding regions with detectable moieties, a hybridization buffer, a labeling buffer with adapters, and a PCR buffer for amplification.
Overall, this patent presents a method and kit for detecting target nucleic acids using hydrogel particles, providing a potential tool for various applications in molecular biology and diagnostics.
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GlobalData, the leading provider of industry intelligence, provided the underlying data, research, and analysis used to produce this article.
GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies
