Agilent Technologies. has been granted a patent for methods and kits in next-generation sequencing. The invention includes sequencing inserts without denaturation, utilizing unlabeled nucleotides, and employing synthesis blocking nucleotides to enhance sequencing accuracy and efficiency. GlobalData’s report on Agilent Technologies gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Agilent Technologies, Microfluidics automation was a key innovation area identified from patents. Agilent Technologies's grant share as of June 2024 was 52%. Grant share is based on the ratio of number of grants to total number of patents.
Next-generation sequencing methods and kits
The patent US12037640B2 outlines a method for sequencing both an insert and an identifier within a polynucleotide construct. The process begins with the annealing of a first primer to a region located 3' to the insert. This primer is then extended by adding labeled nucleotides that are complementary to the insert, allowing for the detection of these nucleotides to ascertain the sequence of the insert. The method also includes the option to extend the double-stranded insert portion by adding unlabeled nucleotides until the complementary strand fully extends over the insert. The polynucleotide construct is designed to include a capture site, primer binding sites, the identifier, and the insert, with specific configurations for the primer binding sites relative to the insert and identifier.
Additionally, the patent describes a more complex sequencing method that involves the annealing of a first primer to a region 3' to the identifier, followed by the addition of labeled nucleotides to determine the identifier's sequence. This method also incorporates the ligation of a strand complementary to the identifier to a second primer before extending it with labeled nucleotides complementary to the insert. The process ensures that the double-stranded identifier portion remains intact during the sequencing of the insert. The claims further detail the use of blocking nucleotides, specifically dideoxy nucleotides, and emphasize the structural arrangement of the polynucleotide construct, which facilitates the sequencing of both the insert and identifier effectively.
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