Danaher has been granted a patent for a method that improves multiplex binding assays by preventing non-specific interactions between dyes. The method involves drying down dye-conjugated reagents in separate locations in a reaction vessel, eliminating erroneous results caused by dyes attaching to each other when dried down together. The patent also includes a claim for a reaction vessel with separate dry reactant spots that do not overlap. GlobalData’s report on Danaher gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Danaher, Laboratory automation robots was a key innovation area identified from patents. Danaher's grant share as of September 2023 was 57%. Grant share is based on the ratio of number of grants to total number of patents.
Multiplex binding assays using separate dye-conjugated reagents
A recently granted patent (Publication Number: US11774443B2) describes a reaction vessel that is designed to receive a liquid sample and facilitate a specific reaction. The vessel includes a first dry reactant spot and a second dry reactant spot, each containing a binding partner conjugated to a dye. These spots are located on different substrates within the vessel and do not overlap. The reaction vessel may also include a divider to separate the binding partners before the liquid sample is added.
The patent also discloses a method for preparing the dry reactant spots. The first reactant is dried onto a membrane substrate to form the first spot, while the second reactant is dried onto a substrate selected from the membrane substrate, a bead, or the inside surface of the vessel to form the second spot. The first and second spots are capable of forming a liquid reaction mixture when contacted with the liquid sample. The first membrane substrate is then placed inside the reaction vessel.
The patent further mentions that the reaction vessel can be in the form of a tube, plate, microtiter plate well, chamber, or slide. The membrane substrates used in the vessel can be made of plastic, nitrocellulose, cellulose acetate, or glass. The dyes used in the reactant spots can be polymer dyes or monomeric dyes. Additionally, the reactant spots or films may contain stabilizers to enhance their stability.
Overall, this patent describes a reaction vessel and method for preparing dry reactant spots that enable specific reactions to occur when a liquid sample is added. The design of the vessel and the use of different substrates for the reactant spots provide flexibility and control in conducting reactions. The patent also highlights the stability of the binding partners and dyes, as well as the various forms the reaction vessel can take.