Singular Genomics Systems has been granted a patent for improved nucleic acid sequencing-by-synthesis (SBS) methods. The patent describes a method of sequencing a DNA molecule using a solid support, primed template DNA molecule, and various dNTP analogue species. The method involves incorporating blocked dNTP analogue species with a polymerase to form a blocked extension product, and imaging the solid support during the process. The entire process takes approximately 1 to 10 minutes. GlobalData’s report on Singular Genomics Systems gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Singular Genomics Systems, DNA polymerase compositions was a key innovation area identified from patents. Singular Genomics Systems's grant share as of September 2023 was 24%. Grant share is based on the ratio of number of grants to total number of patents.
Improved nucleic acid sequencing-by-synthesis method using labeled dntp analogues
A recently granted patent (Publication Number: US11773439B2) describes a method for sequencing DNA molecules. The method involves several steps to accurately determine the nucleotide sequence of a DNA molecule.
In the first step, a solid support containing a primed template DNA molecule is contacted with a deoxyribonucleotide triphosphate (dNTP) analogue species. This dNTP analogue species is complementary to a nucleotide of the template DNA molecule and is associated with a detectable label.
After this initial contact, the template DNA molecule is then exposed to a plurality of blocked dNTP analogue species. These blocked dNTP analogue species are incorporated into the template DNA molecule by a polymerase enzyme, resulting in the formation of a blocked extension product.
Throughout steps (a) and (b), the solid support is imaged. The combined steps (a) and (b) are carried out for a duration of about 1 minute to about 10 minutes.
In some embodiments, after step (a), the dNTP analogue species is removed. Additionally, step (b) involves the addition of at least four different unlabeled, blocked dNTP analogue species.
Following step (c), a blocking moiety linked to a 3'-oxygen atom of the blocked extension product can be removed in step (d) to generate an extendible extension product. The blocking moiety can be selected from various options, including an allyl moiety, a 2-nitrobenzyl moiety, an azido moiety, or a disulfide moiety.
The detectable label associated with the dNTP analogue species is detected in step (c), allowing for the identification of the specific dNTP analogue species. The complement of the identified dNTP analogue species is then assigned as the identity of the nucleotide residue in the template DNA molecule.
The method can be iterated multiple times to determine the nucleotide sequence of at least a portion of the template DNA molecule. In each iteration, the blocked product is treated to generate an extendible extension product, except for the last iteration where this treatment can be omitted.
The solid support used in the method can be made of glass or plastic and may include a polymer. The DNA molecule being sequenced can vary in length, ranging from 50 to more than 250 nucleotides.
Overall, this patented method provides a reliable and efficient approach for sequencing DNA molecules, offering potential applications in various fields such as genomics and molecular biology.