Polymerase Chain Reaction (PCR) is a technic that produces multiple duplicates of a DNA or RNA sample (up to several tens of thousands).
By artificially increasing the number of samples, it simplifies the identification of targeted sequences in the samples. Scientists developed probes to detect these sequences amidst the whole sample but they could not detect sequences present at fewer than 10%.
Droplet-based microfluidic allowed a huge improvement in this technology. With droplet-based microfluidics, it is now possible to detect sequences present at under 0.01%. Numerous applications can benefit from this improvement going from mutation detection in research to cancer monitoring in hospitals.
This significant improvement can only be achieved by using droplets stable through all PCR workflow (2 hours run and frequent changes of temperature up to 95°C), which is where FluoSurf comes in. Its unique features allow it to keep emulsions stable while preserving the precious samples encapsulated.
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