10X Genomics. has been granted a patent for a method that enhances spatial resolution of analytes in biological samples. The technique involves a sandwich system using two substrates with capture probes, enabling precise location determination of multiple analytes through hybridization and spatial barcoding. GlobalData’s report on 10X Genomics gives a 360-degree view of the company including its patenting strategy. Buy the report here.
Smarter leaders trust GlobalData
According to GlobalData’s company profile on 10X Genomics, ChIP-seq sequencing was a key innovation area identified from patents. 10X Genomics's grant share as of July 2024 was 24%. Grant share is based on the ratio of number of grants to total number of patents.
Methods for spatially resolving analytes in biological samples
The granted patent US12071655B2 outlines a method for determining the location of multiple analytes within a biological sample. The process begins with the provision of a first substrate containing a variety of first capture probes, each equipped with a unique spatial barcode and capture domain. A second substrate is then aligned opposite the first substrate, sandwiching the biological sample in between. Prior to this alignment, a reagent medium containing a permeabilization buffer is added to facilitate the migration of intermediate agents and analytes from the biological sample to the substrates. The method involves hybridizing these agents to their respective capture domains and subsequently determining their locations based on the sequences of the spatial barcodes and the intermediate agents.
Further claims detail enhancements to the method, including the use of specific permeabilization buffers, the arrangement of capture probes on beads, and the generation of extended capture probes for amplification. The method is versatile, applicable to various analytes such as RNA and proteins, and can be utilized with different types of biological samples, including formalin-fixed, paraffin-embedded tissues. Additional steps may involve staining or imaging the sample to visualize the analytes. The patent emphasizes the importance of specific binding moieties and capture domains in accurately identifying and locating analytes, thereby contributing to advancements in biological analysis and diagnostics.
To know more about GlobalData’s detailed insights on 10X Genomics, buy the report here.
Data Insights
From
The gold standard of business intelligence.
Blending expert knowledge with cutting-edge technology, GlobalData’s unrivalled proprietary data will enable you to decode what’s happening in your market. You can make better informed decisions and gain a future-proof advantage over your competitors.
GlobalData, the leading provider of industry intelligence, provided the underlying data, research, and analysis used to produce this article.
GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.
