Bio-Rad Laboratories has patented a method for single cell proteomic analysis using high throughput sequencing. The method involves incubating single cells with antibodies conjugated to oligonucleotides, washing away unbound antibodies, and partitioning the cells into mixture partitions for further analysis. GlobalData’s report on Bio-Rad Laboratories gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Bio-Rad Laboratories, Laboratory automation robots was a key innovation area identified from patents. Bio-Rad Laboratories's grant share as of May 2024 was 49%. Grant share is based on the ratio of number of grants to total number of patents.
High throughput proteomic analysis of single cells using sequencing
A recently granted patent (Publication Number: US11965891B2) outlines a method for generating a variety of mixture partitions. The process involves providing fixed and permeabilized single cells, incubating them with a library of structurally distinct antibodies conjugated to epitope-specific oligonucleotides, washing away unbound antibodies, partitioning the cells into mixture partitions, and providing partition-specific oligonucleotides to each partition. These partition-specific oligonucleotides contain unique barcode sequences and primer binding sequences that aid in identifying target epitopes.
Furthermore, the method allows for the use of a library of at least 10, and up to 10,000, structurally distinct antibodies conjugated to epitope-specific oligonucleotides. The process can involve partitioning cells into mixture partitions before providing partition-specific oligonucleotides or vice versa. Additionally, steps are included to discard partitions that do not meet specific criteria, such as not containing a single cell or containing multiple cells. The partition-specific oligonucleotides can be covalently linked to beads with cleavable linkers, and the cells can be lysed during the process. Overall, the method provides a detailed and systematic approach to generating mixture partitions for various applications in the field.
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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.
