Laboratory Corp of America Holdings has been granted a patent for a method to normalize nucleic acid libraries. This method involves using complementary nucleic acid probes to form hybridization complexes, which are then collected on solid supports, allowing for efficient recovery of single-stranded nucleic acids at desired concentrations. GlobalData’s report on Laboratory Corp of America Holdings gives a 360-degree view of the company including its patenting strategy. Buy the report here.
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According to GlobalData’s company profile on Laboratory Corp of America Holdings, Molecular diagnosis biomarkers was a key innovation area identified from patents. Laboratory Corp of America Holdings's grant share as of July 2024 was 44%. Grant share is based on the ratio of number of grants to total number of patents.
Method for normalizing nucleic acid libraries using probes
The granted patent US12071654B2 outlines a method for normalizing nucleic acid libraries, which involves several key steps. Initially, single-stranded nucleic acid molecules from the library are contacted with a solution containing salt and complementary probes that hybridize to specific adaptor sequences (A and B) present on the nucleic acids. These probes are conjugated to a first binding member, which facilitates the formation of hybridization complexes when they bind to solid supports conjugated to a second binding member. The method includes collecting these complexes and separating the nucleic acid molecules from the probes, resulting in a normalized library. The patent also specifies variations of the method, including the use of double-stranded nucleic acids, specific adaptor sequences for next-generation sequencing, and the option to wash the complexes before separation.
Additionally, the patent describes a method for normalizing multiple nucleic acid libraries of varying concentrations, ensuring that the final libraries fall within a narrower concentration range. This involves ligating adaptors to the nucleic acids, denaturing them, and applying a similar amount of probes to each library. The process culminates in the collection and separation of the hybridization complexes, producing normalized libraries suitable for sequencing. The patent also includes a kit designed for this normalization process, comprising the necessary probes and solid supports, thereby facilitating the implementation of the described methods in laboratory settings.
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