Neogen’s patent involves a method for rapid identification of Listeria spp. in environmental samples without the need for an enrichment step. The composition includes a specific lysis reagent solution and an isothermal nucleic acid amplification assay, eliminating the need for enrichment broth. GlobalData’s report on Neogen gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Neogen, Fluorescence flow cytometry was a key innovation area identified from patents. Neogen's grant share as of February 2024 was 62%. Grant share is based on the ratio of number of grants to total number of patents.
Rapid identification of listeria spp. without enrichment broth
A recently granted patent (Publication Number: US11905566B2) discloses a composition for nucleic acid amplification assays and lysis reagent solutions. The composition includes a lysis reagent solution containing specific components such as sodium sulfate, magnesium sulfate, poly(ethylene oxide)octylphenyl ether, potassium phosphate, lysozyme, and proteinase K. Additionally, the composition comprises an isothermal nucleic acid amplification assay involving a polymerase, two template nucleic acids, and a nicking enzyme. Importantly, the composition does not include enrichment broth, distinguishing it from conventional methods.
Furthermore, the patent claims a composition that includes the same lysis reagent solution along with a sample of Listeria, without the presence of enrichment broth. This composition can also incorporate an isothermal nucleic acid amplification assay involving a polymerase, two template nucleic acids, and a nicking enzyme. The patent specifies that the sample in this composition can be an environmental sample, collected using a collection device pre-moistened with letheen broth. These claims highlight the innovative approach of the composition in streamlining nucleic acid amplification assays for efficient and accurate detection of target nucleotide sequences, particularly in environmental samples.
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