Agilent Technologies patented a Polymerase Chain Reaction (PCR) primer with a specific structure for use in nucleic acid amplification reactions. The primer includes components like a fluorescence resonance energy transfer (FRET) pair and a double-stranded restriction enzyme recognition site. GlobalData’s report on Agilent Technologies gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Agilent Technologies, Microfluidics automation was a key innovation area identified from patents. Agilent Technologies's grant share as of February 2024 was 54%. Grant share is based on the ratio of number of grants to total number of patents.

Pcr primer with specific structure for nucleic acid amplification

Source: United States Patent and Trademark Office (USPTO). Credit: Agilent Technologies Inc

A recently granted patent (Publication Number: US11926870B2) discloses a Polymerase Chain Reaction (PCR) primer with a specific structure, including a double-stranded restriction enzyme recognition site and a fluorescence resonance energy transfer (FRET) pair. The primer is designed with a 5 to 15 bp PspG1 restriction enzyme recognition sequence, enhancing its specificity. Additionally, the FRET pair includes various fluorophores like carboxyfluorescein and quenchers, enabling efficient PCR amplification.

The patent also covers a method for performing PCR, particularly quantitative PCR (qPCR), using the described primer and reagents. The method involves amplifying a template with the specific PCR primer structure and utilizing the PspG1 restriction enzyme. Notably, the method allows for the amplification of multiple amplicons in a single nucleic acid amplification reaction, with options to amplify at least 4, 6, or 10 amplicons. This innovation in PCR technology could have significant implications in various fields requiring precise and efficient nucleic acid amplification techniques.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.