Bio-Techne‘s patent involves a method for sizing repeat regions of nucleic acids using capillary electrophoresis and internal/external sizing standards. The system utilizes ladder amplification products to determine nucleic acid size accurately. GlobalData’s report on Bio-Techne gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Bio-Techne, Peptide pharmacophores was a key innovation area identified from patents. Bio-Techne's grant share as of February 2024 was 43%. Grant share is based on the ratio of number of grants to total number of patents.

Method for sizing nucleic acid repeat regions using electrophoresis

Source: United States Patent and Trademark Office (USPTO). Credit: Bio-Techne Corp

The granted patent (Publication Number: US11913068B2) discloses a method for sizing a repeat region of a nucleic acid. The method involves providing a nucleic acid sample, amplifying a nucleic acid with a repeat region, performing capillary electrophoresis to obtain a ladder of amplification products, and generating an internal sizing standard. This standard is created by identifying repeat profiles, generating repeat peak locations, and estimating a linear relationship between peak location and fragment size. The repeat region is sized using this internal standard along with an external sizing standard, which is generated by identifying peaks in the ladder of amplification products and combining linear and non-linear relationships.

Furthermore, the method includes mobility-corrected sizing standards, iterative re-estimation of peak locations, and piecewise scaling of the background model. It also involves determining gene-specific peak locations in the repeat profile based on dynamic thresholds and amplitude criteria. The method aims to provide an accurate and reliable way to size repeat regions of nucleic acids, offering a comprehensive approach that combines internal and external sizing standards, iterative processes, and gene-specific peak identification for enhanced accuracy and efficiency in nucleic acid analysis.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.