Repligen has filed a patent for a system that enables TFF filtration of a feedstock. The system includes a feed vessel, filter membrane, feed pump, buffer vessel, and a diafiltration pump. It also incorporates a variable path-length instrument to determine the concentration of the target biomolecule in real time. A controller is used to control the operation of the system based on the received information. GlobalData’s report on Repligen gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Repligen, rtRNA virus peptides was a key innovation area identified from patents. Repligen's grant share as of September 2023 was 43%. Grant share is based on the ratio of number of grants to total number of patents.

A system for tff filtration of a feedstock

Source: United States Patent and Trademark Office (USPTO). Credit: Repligen Corp

A recently filed patent (Publication Number: US20230294021A1) describes a system and method for filtration of a feedstock containing a target biomolecule. The system includes a feed vessel, a filter membrane, a feed pump, a permeate line, a variable path-length instrument, and a controller. The filter membrane is coupled to the feed vessel via a feed line and a retentate line, allowing for the filtration of the solution and the return of filtered solution to the feed vessel. The feed pump is responsible for moving the solution from the feed vessel to the filter membrane. The permeate line is used to deliver permeate from the filter membrane to a permeate vessel. The variable path-length instrument is capable of transmitting light through the solution in the feed line and correlating the transmitted light with the concentration of the target biomolecule in real-time. The controller receives information from the variable path-length instrument and the feed pump, and based on this information, it executes instructions to control the operation of the feed pump, the diafiltration pump, and the variable path-length instrument.

The controller is programmed to adjust the speed of the diafiltration pump to maintain the concentration of the target biomolecule within a predetermined range. It can also receive input values from a user for the target concentration and/or diafiltration volume, allowing for a combination of concentration and diafiltration steps. The controller periodically determines whether the concentration of the target biomolecule is within the user-specified range or equal to the user input final concentration, based on information received from the variable path-length instrument. If the concentration departs from the user-specified range or value by a predetermined amount, the controller can provide a user-alert.

The system also includes a buffer vessel and a diafiltration pump for delivering a buffer solution to the feed vessel. A backpressure control valve is disposed in the retentate line to control the transmembrane pressure of the filter membrane. Pressure sensors in the feed line, retentate line, and permeate line are used to measure feed, retentate, and permeate pressures, respectively, and determine the transmembrane pressure.

The method described in the patent involves delivering a solution containing the target biomolecule to the filter membrane, returning the retentate portion to the feed vessel, and delivering the permeate portion to a permeate vessel. The concentration of the target biomolecule is determined in real-time using the variable pathlength instrument. Based on this determination, various actions can be performed, such as continuing the filtration process, adding a buffer solution, providing an alert to the user, or stopping the method. The method can also involve a diafiltration process, where a user-defined diafiltration volume, diafiltration concentration, and final concentration are received at a user interface. The diafiltration process continues until the user-selected diafiltration volume is achieved, and a buffer solution can be added to maintain a constant diafiltration concentration. Once the user-selected final concentration is reached, a recirculation mode is initiated to maintain the retentate portion at a constant concentration.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies