Sysmex has patented a method for classifying and counting leukocytes in biological samples. This method enables the differentiation between normal leukocytes, atypical lymphocytes, and myeloblasts using a specific reagent kit and flow cytometry techniques to analyze fluorescence and light scattering. GlobalData’s report on Sysmex gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Sysmex, Laboratory automation robots was a key innovation area identified from patents. Sysmex's grant share as of July 2024 was 48%. Grant share is based on the ratio of number of grants to total number of patents.

Method for classifying and counting leukocytes in samples

Source: United States Patent and Trademark Office (USPTO). Credit: Sysmex Corp

The granted patent US12072330B2 outlines a method for detecting atypical lymphocytes or myeloblasts in biological samples. The process begins with the preparation of a measurement sample, which involves mixing the biological sample with a first reagent containing a fluorescent dye for nucleic acid staining and a second reagent composed of cationic and nonionic surfactants along with an aromatic organic acid. This second reagent must have a concentration between 30 mM and 50 mM and a pH ranging from 5.5 to 7.0. Following the preparation, the sample is analyzed using a flow cytometer to obtain side-scattered light intensity and fluorescence intensity. The resulting data is then plotted on a scattergram, allowing for the classification of monocytes and lymphocytes, and the identification of clusters indicative of atypical lymphocytes or myeloblasts.

Further claims specify the composition of the second reagent, indicating that the aromatic organic acid can be selected from various types, including aromatic carboxylic acids and aromatic sulfonic acids. The concentration of this acid is preferably between 40 mM and 50 mM, with an optimal pH of 5.5 to 6.2. The method emphasizes the importance of side-scattered light information for distinguishing atypical lymphocytes, which deviate towards lymphocyte clusters, and myeloblasts, which deviate towards monocyte clusters on the scattergram. Additionally, the patent details the types of surfactants used, including quaternary ammonium salt surfactants or pyridinium salt surfactants for cationic surfactants, and polyoxyethylene nonionic surfactants for nonionic surfactants, further refining the method's specificity and effectiveness in identifying these atypical cells.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.